Establishment of IDA model group (IDAG)
- 5-week-old female Wistar rats
- Received an 8-week treatment with low iron diet, deionized water and weekly bloodletting
- With haemoglobin content <90 g/L were considered as IDA
- Subsequently mated with 5-week-old male Wistar rats
Intervention
- After pregnancy, rats in IDAG were fed diets containing 3 different iron supplements until offspring turned 42 days old
- Ferrous sulfate (FS)
- Iron protein succinylate (IPS)
- Iron polysaccharide complex (IPC)
- Randomly divided into 7 groups except normal control group (NG)
- IDAG
- Low dose of IPC at iron dose of 30 mg·kg−1 (IPC-L)
- High dose of IPC at iron dose of 50 mg·kg−1 (IPC-H)
- Low dose of IPS at iron dose of 30 mg·kg−1 (IPS-L)
- High dose of IPC at iron dose of 50 mg·kg−1 (IPS-H)
- Low dose of FS at iron dose of 30 mg·kg−1 (FS-L)
- High dose of FS at iron dose of 50 mg·kg−1 (FS-H)
Effects of iron supplementation on maternal rats
(When compared to those in IDAG)
- Significantly improved pregnancy outcomes
- Improved immune-inflammatory status which has been altered by IDA
- Increased levels of serum iron (SI), transferrin saturation (TS) and serum ferritin (SF)
- Significantly decreased level of total iron binding capacity (TIBC)
- Iron stores in the liver and spleen increased upon iron supplementation
Effects of iron supplementation on offspring
(When compared to those in IDAG)
- The levels of HGB, HCT, MCV, CHCM and CH# were higher than those in IDAG
- The level of RDW# was significantly lower
- The immune-inflammatory status of offspring of IDA rats was improved
- The onset time of surface righting reflex, cliff avoidance, negative geotropism and air righting reflex was significantly earlier, with no significant difference with NG
- Improved spatial learning ability which has been affected by IDA
- Improved abnormal and retarded neurodevelopment of IDA offspring by increasing protein expression of SYN1, NMDAR and PSD-95
#HGB = haemoglobin; HCT = hematocrit; MCV = mean corpuscular volume; CHCM = cell haemoglobin concentration mean; CH = haemoglobin content of red blood cell; RDW = red cell volume distribution width
Link to the full article:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809104/pdf/41598_2020_Article_79971.pdf
Reference:
Zhang Q et al. Adverse effects of iron deficiency anemia on pregnancy outcome and offspring development and intervention of three iron supplements. Sci Rep. 2021;11(1):1347.
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