Growth of isolated bacterial strains
- Bifidobacterium infantis Y538 completely utilized all 5 tested HMOs
- Bifidobacterium dentium Y510 and Y521 partially utilized LNT and LNnT only, at a relatively low efficiency
- Only galactose moiety was degraded
- Lacto-N-triose (LNTII) moiety was released
- Other 9 strains did not grow significantly on the tested HMOs compared to the controls without carbon source
Two β-Galactosidases characterized from Bifidobacterium dentium Y510
- Both acted intracellularly
- β-Galactosidase Bdg42A hydrolyzed LNT into galactose and LNTII
- β-Galactosidase Bdg2A hydrolyzed type-2 oligosaccharides such as lactose and LNnT
* 2’-fucosyllactose (2’-FL), 3-fucosyllactose (3-FL), Difucosyllactose (DFL), lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT)
Link to the full article:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639736/pdf/41598_2021_Article_2741.pdf
Reference:
Moya-Gonzálvez EM et al. Infant-gut associated Bifidobacterium dentium strains utilize the galactose moiety and release lacto-N-triose from the human milk oligosaccharides lacto-N-tetraose and lacto-N-neotetraose. Sci Rep. 2021;11(1):23328.
Other articles that you might be interested in:
[Literature library] Human milk oligosaccharide concentration throughout lactation
WYE-EM-215-DEC-21